Swimming Behavior of Pseudomonas aeruginosa Studied by Holographic 3D Tracking

نویسندگان

  • Svenja M. Vater
  • Sebastian Weiße
  • Stojan Maleschlijski
  • Carmen Lotz
  • Florian Koschitzki
  • Thomas Schwartz
  • Ursula Obst
  • Axel Rosenhahn
چکیده

Holographic 3D tracking was applied to record and analyze the swimming behavior of Pseudomonas aeruginosa. The obtained trajectories allow to qualitatively and quantitatively analyze the free swimming behavior of the bacterium. This can be classified into five distinct swimming patterns. In addition to the previously reported smooth and oscillatory swimming motions, three additional patterns are distinguished. We show that Pseudomonas aeruginosa performs helical movements which were so far only described for larger microorganisms. Occurrence of the swimming patterns was determined and transitions between the patterns were analyzed.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Genetic diversity of Pseudomonas aeruginosa strains isolated from ventilated patients with nosocomial pneumonia, cancer patients with bacteremia, and environmental water.

Random amplified polymorphic DNA typing was used to study the genetic diversity of Pseudomonas aeruginosa strains from (i) ventilated patients with nosocomial pneumonia who were hospitalized in intensive care units, (ii) cases of bacteremia in cancer patients with severe neutropenia, and (iii) rivers and swimming pools. Genetic diversity was determined by three phylogenetic methods and by stati...

متن کامل

Effects of Sub-inhibitory Concentrations of Essential Oils of Mentha spicata and Cumminum cyminum on Virulence Factors of Pseudomonas aeruginosa

  Background : Pseudomonas aeruginosa is one of important opportunistic pathogen, that cases serious infections . It produces many virulence factors, and this bacterium usually is resistance against antimicrobial agents.   Objective :The aim of this study was evaluate the effects of sub-MICs of essential oils of Mentha spicata and Cumminum cyminum on alginate production, biofilm formation, swim...

متن کامل

ارزیابی شاخص‌های بهداشتی کیفیت آب استخرهای شنای شهر ارومیه در سال 1392

Background and Objective: Contamination of swimming pools’ water can lead to the transfer of different diseases to humans. The aim of this study was to investigate the water quality health indicators in Urmia swimming pools. Materials and Methods: In this cross-sectional study, 14 active covered swimming pools in Urmia city were chosen as census. The physical parameters such as pH, tem...

متن کامل

Hyperswarming adaptations in a bacterium improve collective motility without enhancing single cell motility.

Pseudomonas aeruginosa is a monoflagellated bacterium that can use its single polar flagellum to swim through liquids and move collectively over semisolid surfaces, a behavior called swarming. Previous studies have shown that experimental evolution in swarming colonies leads to the selection of hyperswarming bacteria with multiple flagella. Here we show that the advantage of such hyperswarmer m...

متن کامل

Tracking E. coli runs and tumbles with scattering solutions and digital holographic microscopy.

We use in-line digital holographic microscopy to image freely swimming E. coli. We show that fitting a light scattering model to E. coli holograms can yield quantitative information about the bacterium's body rotation and tumbles, offering a precise way to track fine details of bacterial motility. We are able to extract the cell's three-dimensional (3D) position and orientation and recover beha...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 9  شماره 

صفحات  -

تاریخ انتشار 2014